A NOVEL HYDRODYNAMIC FOCUSING MICRODEVICE FOR FLOW CYTOMETRY APPLICATIONS

Hydrodynamic focusing is one of the most utilized techniques in microfluidics. Its
applications have been employed in a wide variety of biological analyses including on-chip flow
cytometry, single molecule detection, and laminar mixers. In the present study, a new
hydrodynamic focusing microdevice for flow cytometry applications is presented and numerically
investigated. In the proposed microdevice, the sample fluid is compressed both in vertical and
horizontal directions simultaneously by radial sheath flows injected within flow cytometer through
one ring. The microdevice configuration is optimized and effective parameters on stream
distribution are investigated. In addition, in order to observe hydrodynamic focusing phenomena,
particles trajectories are studied. Moreover, results of the proposed model are compared with the
previous one in order to verify that the performance of the present model is more efficient.